The rate of turnover in a metabolic pathway, otherwise known as the metabolic flux , is regulated based on the stoichiometric reaction model, the utilization rate of metabolites, and the translocation pace of molecules across the lipid bilayer .  The regulation methods are based on experiments involving 13C-labeling , which is then analyzed by Nuclear Magnetic Resonance (NMR) or gas chromatography-mass spectrometry (GC-MS) -derived mass compositions. The aforementioned techniques synthesize a statistical interpretation of mass distribution in proteinogenic amino acids to the catalytic activities of enzymes in a cell. 
For example, an enlarged, hyperechoic liver is brighter than the spleen. This can be caused by steroid administration, diabetes, or several other diseases. If there are nodules or masses that are hypoechoic to normal liver, hyperechoic, or mixed, we know that there are focal lesions but not what they are. The description helps a reader to “see” the lesion, but may not be specific to a particular disease. A hypoechoic lesion could be benign liver hyperplasia, which is very common in older dogs, or a cancerous nodule. Certain patterns, such as a “target” lesion, are more associated with cancer. If the diagnosis is unclear after ultrasound, a fine needle aspirate or biopsy might be recommended to determine what the nodule is.
When he came to visit me once again in November of 2011, he was no longer able to walk into my office and he was in a wheel chair. He begged me to help him. See, Mr. Daniels has a zest for life, and he was not happy with the hand he was currently dealt. He is a very accomplished musician and he wanted to live life on his terms and not on the terms his health was dishing out to him. I told him that if he was willing to make some lifestyle changes that were dramatic immediately, that I might be able to help him. I told him that I needed him to eat a strict ketogenic Epi-paleo diet right away, and that he had to begin to ice his lower back and thighs for me daily. He told me the request sounded odd, but that if I was going to try to help him he would do anything at this point because no one else had any answers for him.